DEVELOPMENT OF A TEST SYSTEM FOR DETERMINING THE CONCENTRATION OF RABIES VIRUS RIBONUCLEOPROTEIN IN RAW MATERIALS FOR VACCINES USING ELISA

Authors

  • M. Doronin Federal State Budgetary Institution "Federal Center for Animal Health Protection"

Keywords:

rabies virus; ribonucleoprotein concentration; ELISA.

Abstract

 The purpose of the article is to develop a test system for determining the concentration of rabies virus ribonucleoprotein in raw materials for vaccines using ELISA. Methods of conjugation of immunoglobulin G with horseradish peroxidase were used, a direct variant of ELISA was performed, and diagnostic capabilities of the test system were determined. As a result, a test system was created to determine the concentration of analyte in raw materials for vaccines, a mathematical model was revealed between the extinction in ELISA and the concentration of ribonucleoprotein, and the high diagnostic accuracy of the developed test system was proved.

Author Biography

M. Doronin , Federal State Budgetary Institution "Federal Center for Animal Health Protection"

candidate of Biological Sciences, doctoral student 

References

Andrers Albom, Stefan Norrel. Vvedenie v sovremennuyu epidemiologiyu / Mati Rahu; per. s angl. I. Bonya – Tallin: In-t eksperim. i klin. mediciny (Estoniya), Dat. protivorak. ovo., 1996. –122 s.

Gruzdev K.N., Metlin A.E. Beshenstvo zhivotnyh. – Vladimir: FGBU «VNIIZZh», 2019. – 394 s.

Egorov A.M., Osipov A.P., Dzantiev B.B. Teoriya i praktika immunofermentnogo analiza – M.: Vyssh. shk., 1991. – 288 s.

Bradford, M. M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding // Anal. Biochem. – 1976. – V. 72. – P. 248-254.

OIE. Manual of diagnostic tests and vaccines for terrestrial animals. – Paris, 2018. – Chapter 3.1.17. – P. 578-612.

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Published

2021-11-22

Issue

Vol. 3 No. 72 (2021)

Section

Статьи

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